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培养箱中微生物菌种保藏方法及注意点
发布时间:2019-12-13   点击次数:75次

 1、传代保存法:有些微生物当遇到冷冻或干燥等处理时,会很快死亡,因此在这种情况下,只能求助于传代培养保存法。传代培养就是要定期地进行菌种转接、培养后再保存,它是基本的微生物保存法,例如酸奶等常用生产菌种的保存。

 

  CHUANDAIBAOCUNSHI,PEIYANGJIDENONGDUBUYIGUOGAO,YINGYANGCHENGFENBUYIGUOYUFENGFU,YOUQISHITANSHUIHUAHEWUDENONGDUYINGZAIKENENGDEFANWEINEIJINLIANGJIANGDI。PEIYANGWENDUTONGCHANGYISHAODIYUSHISHENGZHANGWENDUWEIHAO。RUOWEICHANSUANJUNZHONG,ZEYINGZAIPEIYANGJIZHONGTIANJIASHAOLIANGTANSUANGAI。

 

众赢彩票注册  YIBANDI,DADUOSHUJUNZHONGDEBAOZANGWENDUYI5℃WEIHAO,XIANGYANYANGJUN、HUOLUANHUJUNJIBUFENBINGYUANZHENJUNDENGWEISHENGWUJUNZHONGZEKEYISHIYONG37℃JINXINGBAOCUN,ERXUNLEIDENGDAXINGSHIYONGJUNDEJUNZHONGZEKEYISHIWENZHIJIEBAOCUN。

 

  CHUANDAIPEIYANGBAOCUNFASUIRANJIANBIAN,DANQIQUEDIANYEHENMINGXIAN,RU:①JUNZHONGGUANMIANSAIJINGCHANGRONGYIFAMEI;②JUNZHUDEYICHUANXINGZHUANGRONGYIFASHENGBIANYI;③FANFUCHUANDAISHI,JUNZHUDEBINGYUANXING、XINGCHENGSHENGLIHUOXINGWUZHIDENENGLIYIJIXINGCHENGBAOZIDENENGLIDENGJUNYOUJIANGDI;④XUYAODINGQIZHUANZHONG,GONGZUOLIANGDA;⑤ZAJUNDEWURANJIHUIJIAODUO。

 

众赢彩票注册  2、YETISHILAFUGAIBAOCUNFA:GAIFAJIAOQIANYIZHONGFANGFABAOCUNJUNZHONGDESHIJIANGENGZHANG,SHIYONGYUMEIJUN、JIAOMUJUN、FANGXIANJUNJIXUYANGXIJUNDENGDEBAOCUN。CIFAKEFANGZHIGANZAO,BINGTONGGUOXIANZHIYANGDEGONGJIERDADAOXUERUOWEISHENGWUDAIXIEZUOYONGDEMUDE。QIJUYOUFANGFAJIANBIANDEYOUDIAN,TONGSHIYESHIYONGYUBUYILENGDONGGANZAODEWEISHENGWU(RUCHANBAONENGLIDIDESIZHUANGJUN)DEBAOCUN,ERMOUXIEXIJUNRUGUDANJUN、RUSUANGANJUN、MINGCHUANZHUJUN、FENZHIGANJUN、HONGLUOJUNJISHAMENSHIJUNDENGHEYIXIEZHENJUNRUJUANMEIJUN、XIAOKEYINHANMEI、MAOMEI、GENMEIDENGBUYICAIYONGCIFAJINXINGBAOCUN。

 

众赢彩票注册  3、ZAITIBAOCUNFA:JIJIANGWEISHENGWUXIFUZAISHIDANGZAITISHANGJINXINGGANZAOBAOCUNDEFANGFA。CHANGYONGDEYOUFANGFABAOKUOYIXIAJIZHONG,RU①TURANGBAOCUNFA:ZHUYAOYONGYUNENGXINGCHENGBAOZIHUOBAONANGDEWEISHENGWUJUNZHONGDEBAOZANG。FANGFASHIZAIMIEJUNDETURANGZHONGJIARUJUNYE,LIJIZAISHIWENXIAJINXINGGANZAOHUOSHIJUNTIFANZHIHOUZAIGANZAO,RANHOULENGZANGHUOZAISHIWENXIAMIFENGBAOCUN。BAOCUNYONGDETURANGYUANZESHANGYIFEIWODEGENGTUWEIYI,TURANGXUFENGGAN、FENSUI、GUOSHAIHEMIEJUN。

 

  SHIWEISHENGWUZAITURANGZHONGFANZHIHOUJINXINGGANZAOBAOCUNDEFANGFASHI:QUSHILIANGTURANG(5KE)ZHIYUSAIYOUMIANSAIDESHIGUANZHONG,JIASHUIHUOJIARUCHONGFENXISHIDEYETIPEIYANGJI(YIHANSHUILIANGWEITURANGDACHISHUILIANGDE60%WEIYI),RANHOUGAOYAMIEJUN。ZAIJIANGXUBAOCUNDEWEISHENGWUJINXINGDALIANGJIEZHONG,PEIYANGZHIJUNSINENGYONGROUYANQUERENDECHENGDUWEIZHI,YIRUGANZAOQIZHONGJINGDUANSHIJIANGANZAOHUOFENGGANHOUMIFENG,LENGZANGHUOSHIWENBAOCUN。

 

  ②SHATUBAOCUNFA:QUQINGJIEDESHA,GUO60MUSHAIQUDIAODASHALI,BINGYONGCITIEXIQUSHAZHONGTIEXIE,ZAIYONGNaOHRONGYE、10%HClRONGYEHESHUIJIAOTIQINGXISHUCI,GANZAOHOU,ZHIYUSHIGUANHUOANPINGGUANZHONGBAOCHI2~3cmSHEN,ZAIJINGGANREMIEJUNHOU,JIARU1mlJUNZHONGPEIYANGYE,JINGCHONGFENHUNYUNHOU,FANGRUZHENKONGGANZAOQIZHONG,WANQUANGANZAOHOURONGFENGBAOCUN。YEKEYONGERFENXIJINGDESHA(JINGHClYUCHULI)HEYIFENPINJI、GUOSHAIDEHUANGTUCHANHEHOUMIEJUN,ZAIJINXINGJUNZHONGBAOZANG。

 

  ③GUIJIAOBAOCUNFA:YI6~16MUDEWUSEGUIJIAODAITISHAZI,GANREMIEJUNHOU,JIARUJUNYE。JIAJUNYESHI,YOUYUGUIJIAODEXIFURECHANGSHIWENDUSHENGGAO,YINERXUSHEFAJIAYILENGQUE。

 

众赢彩票注册  ④CIZHUBAOCUNFA:JIANGJUNYEJINRUSUSHAOCIZHU(HUODUOKONGBOLIZHU)HOUZAIJINXINGGANZAOBAOCUNDEYIZHONGFANGFA。ZAILUOXUANKOUSHIGUANZHONGZHUANGRU1/2GUANGAODEGUIJIAO(HUOWUSHUICaSO4),SHANGPUBOLIMIAN,ZAIFANGSHANG10~20LICIZHU,JINGGANREMIEJUNHOU,JIERUJUNXUANYE,HOULENGZANG、SHIWENBAOZANGHUOJIANYAGANZAOHOUMIFENGBAOZANG。BENFADUIJIAOMUJUNHENYOUXIAO,TEBIESHIYONGYUGENLIUJUN,KEBAOCUNZHANGDALIANGNIANBANSHIJIAN。

 

众赢彩票注册  ⑤FUPIBAOCUNFA:ZAIFUPINEIJIARU60%DESHUI,JINGMIEJUNHOUJIEZHONGPEIYANG,HOUGANZAOBAOZANG。

 

  ⑥ZHIPIAN(LVZHI)BAOCUNFA:JIANGMIEJUNZHIPIANJINRUPEIYANGYEHUOJUNXUANYEZHONG,CHANGYAHUOJIANYAGANZAOHOU,ZHIYUZHUANGYOUGANZAOJIDERONGQINEIJINXINGBAOCUN。

 

众赢彩票注册  4、XUANYEBAOCUNFA:JISHIWEISHENGWUHUNXUANYUSHIDANGRONGYEZHONGJINXINGBAOCUNDEFANGFA。CHANGYONGDEYOU,①ZHENGLIUSHUIBAOCUNFA:SHIYONGYUMEIJUN、JIAOMUJUNJIJUEDABUFENFANGXIANJUN,JIANGQIJUNTIXUANFUYUZHENGLIUSHUIZHONGJIKEZAISHIWENXIABAOCUNSHUNIAN。BENFAYINGZHUYIBIMIANSHUIFENDEZHENGFA。

 

众赢彩票注册  ②TANGYEBAOCUNFA:SHIYONGYUJIAOMUJUN,RUJIANGQIJUNTIXUANFUYU10%DEZHETANGRONGYEZHONG,RANHOUYULENGANCHUBAOCUN,KEZHANGDA10NIAN。CHUCIZHIWAI,YEKESHIYONGHUANCHONGYEHUOSHIYANSHUIDENGJINXINGBAOCUN。

 

  5、JIZHUBAOCUNFA:JILINGWEISHENGWUQINRUQIJIZHUHOUJIAYIBAOCUNDEFANGFA。

 

  6、LENGDONGBAOCUNFA:SHIYONGYUKANGDONGLIQIANGDEWEISHENGWU。ZHEIXIEWEISHENGWUKEZAIQIJUNTIXIBAOWAIZAOSHOUDONGJIEDEQINGKUANGXIAERBUSHOUSUNSHANG,ERDUIQITADADUOSHUWEISHENGWUERYAN,WULUNZAIXIBAOWAIDONGJIEHAISHIZAIXIBAONEIDONGJIE,DOUHUIDUIJUNTIZAOCHENGSUNSHANG,YINCIDANGCAIYONGZHEIZHONGBAOZANGFANGFASHI,YINGZHUYIYIXIAJIDIAN,①YAOXUANZESHIYULENGDONGGANZAODEJUNLINGXIBAO;②YAOXUANZESHIYIDEPEIYANGJI,YINWEIMOUXIEWEISHENGWUDUILENGDONGDEDIKANGLI,CHANGSUIPEIYANGJICHENGFENDEBIANHUAERXIANSHICHUJUDACHAYI;③YAOXUANZEHESHIDEJUNYENONGDU,TONGCHANGJUNYENONGDUYUEGAO,SHENGCUNLVYUEGAO,BAOCUNQIYEYUEZHANG;④ZAIJUNYENEIBUTIANJIADIANJIEZHI(RUSHIYANDENG);⑤KEZAIJUNYENEITIANJIAGANYOUDENGBAOHUJI,YIFANGZHIZAILENGDONGGUOCHENGZHONGCHUXIANJUNTIDALIANGSIWANGDEXIANXIANG。TONGYANG,YEKETIANJIAGEZHONGTANGLEI、QUXIANWEIXUEYEHETUOZHINIURUDENGJUYOULIANGHAOBAOHUXIAOGUODERONGJI,DANDUIYOUXIEWEISHENGWUERYAN,BUJIABAOHUJISHIGENGYOUXIAO。

 

众赢彩票注册  ⑥YUANZESHANGYINGJINKUAIJINXINGLENGDONGCHULI,DANDANGJIARUBAOHUJISHI,KEJINGZHIYIDUANSHIJIANHOUZAIJINXINGCHULI。

 

  ⑦JIUDONGWUXIBAOERYAN,YINGZAI-20℃FANWEINEIYI1℃/minZUOYOUDESUDUHUANMANJIANGWEN,CIHOUBIXUJINKUAIJIANGDAOZHUZANGWENDU;ERDUIJUEDADUOSHUWEISHENGWUERYAN,ZEBUBIRUCI,RUJIEGOUJIAOWEIFUZADEYUANCHONGZEKEZAI-35℃FANWEINEIJINXINGHUANMANJIANGWEN,ERSHIJUNTIZEBIXUCAIYONGSHANGSHUDEERJIEDUANFAJINXINGLENGDONG;⑧RUOJINXINGZHANGQIBAOCUN,ZEZHUZANGWENDUYUEDIYUEHAO;⑨QUYONGLENGDONGBAOCUNDEJUNZHONGSHI,YINGCAIQUSURONGCUOSHI,JIZAI35~40℃WENSHUIZHONGQINGQINGZHENDANGSHIZHIXUNSURONGJIE。ERJIUYANYANGJUNLAISHUO,ZEYINGXUANZEJINGZHIRONGHUADECUOSHI。DANGLENGDONGJUNRONGHUAHOU,YINGJINLIANGBIMIANZAICILENGDONG,FOUZEJUNTIDECUNHUOLVJIANGXIANZHUXIAJIANG。

 

众赢彩票注册  CHANGYONGDELENGDONGBAOCUNFABAOKUO:

 

  ①DIWENBINGXIANGBAOCUNFA(-20℃、-50℃HUO-85℃):DIWENLENGDONGBAOCUNSHISHIYONGLUOXUANKOUSHIGUANJIAOWEIFANGBIAN,YEKEZAIMIANSAISHIGUANWAIBAOGUOSULIAOBOMO。BAOCUNSHIJUNYEJIALIANGBUYIGUODUO,YOUXIEKETIANJIABAOHUJI。CIWAI,YEKEYONGφ5mmDEBOLIZHULAIXIFUJUNYE,RANHOUBABOLIZHUZHIYUSULIAORONGQINEI,ZAIFANGRUDIWENBINGXIANGNEIJINXINGBAOCUNDE。

 

  ②GANBINGBAOCUNFA(-70℃ZUOYOU):JIJIANGJUNZHONGGUANCHARUGANBINGNEI,ZAIZHIYUBINGXIANGNEIJINXINGLENGDONGBAOCUN。

 

  ③YEDANBAOCUNFA(-196℃):SHISHIYONGFANWEIGUANGDEWEISHENGWUBAOCUNFA。QICAOZUOBUZHOURUXIA,a、ZHUANGANPINGGUAN:SHIYONGJINLIANGNONGHOUDEJUNTIXUANFUYUHANYOUSHIDANGFANGDONGJI(BAOCUNMEIJUNBUYONGFANGDONGJI)DEMIEJUNRONGYEZHONG,JIANG0.2~1mlDEZHEIZHONGRONGYEFENZHUANGYUANPINGZHONG,HUOZAIZHUANGYOUFENSANJIDEANPINGZHONGZHIJIEJIEZHONG,HUOJIANGJUNSITIQIONGZHIKUAIZHIJIEXUANFUYUFENSANJIZHONG。

 

众赢彩票注册  b、RONGFENGANPINGGUAN:RUOZHIJIEZHUCUNYUQIXIANGYEDANZHONG(-150℃~-170℃)SHI,ZEBUXURONGFENG。

 

  c、JIANCHAANPINGGUANSHIFOURONGFENGLIANGHAO:JIZAI4℃XIA,JIANGRONGFENGANPINGGUANZAISHIDANGDESESURONGYEZHONGJINPAO2~30FENZHONGHOU,GUANCHAYOUWUSESUJINRUANPING。

 

众赢彩票注册  d、HUANMANLENGQUE:JIANGRONGFENGANPINGGUANZHIYUXIAOGUANZHONG,RANHOUYONGYEDANQIYIYUE1℃/minDESUDULENGQUEZHI-25℃ZUOYOU,YEKEZAI-20℃~-25℃DEBINGXIANGNEIHUANMANLENGQUE30~60min。

 

众赢彩票注册  e、SULENG:HOUJINRUYEDANZHONGKUAISULENGQUEZHI-196℃。

 

  7、LENGDONGGANZAOBAOCUNFA:QIYUANLISHISHOUXIANJIANGWEISHENGWULENGDONG,RANHOUZAIJIANYAXIALIYONGSHENGHUAXIANXIANGCHUQUSHUIFEN。SHISHISHANG,CONGJUNTIZHONGCHUQUDABUFENSHUIFENHOU,XIBAODESHENGLIHUODONGJIUHUITINGZHI,YINCIKEYIDADAOZHANGQIWEICHISHENGMINGZHUANGTAIDEMUDE。GAIFANGFASHIYONGYUJUEDADUOSHUWEISHENGWUJUNZHONG(BAOKUOSHIJUNTIHELIKECISHITIDENG)DEBAOCUN。

 

众赢彩票注册  ZAIJINXINGLENGDONGGANZAOSHI,XUZHUYIYIXIAJIGEWENTI:

 

  a、LENGDONGGANZAOQIANDEPEIYANGTIAOJIAN:SHOUXIANJIANYANJUNZHONGCHUNDU。YIBANDISHUO,JIANGDAIBAOCUNDEWEISHENGWUZAIYINGYANGFENGFUQIERONGYIZENGJUNDEPEIYANGJISHANGJINXINGPEIYANGWEIYI;JUNLINGYIDADAODUISHUSHENGZHANGQIWEIHAO;RUOWEIYOUYABAOHUOBAOZIDEWEISHENGWU,ZEYIYABAOHEBAOZIXINGCHENGYIHOUJINXINGBAOCUNWEIHAO;JUNYENONGDUYIGAOWEIHAO,RUXIJUNYINGDADAO109~1010/ml。

 

众赢彩票注册  b、JUNZHUHAOMADENGDEBIAOJI:KEZAIANPINGWAICEBIAOJIHUOZAIANPINGNEIFENGRUBIAOQIAN。

 

  c、ANPINGDEZHUNBEI:JIANGANPINGZAI2%HClZHONGJINPAOGUOYE,ZILAISHUICHONGXI3CIHOU,ZHENGLIUSHUISHUAJING,GANZAO,SAIMIANSAI,TIEBIAOQIAN,GANREMIEJUNHUOWENREMIEJUNHOU,60℃HENGWENGANZAO。

 

  d、TIANJIABAOHUJI:CHANGYONGDEBAOHUJIYOUTUOZHIRU、12%ZHETANG、JIA7.5%PUTAOTANGDEPUTONGROUTANGYIJIJIA7.5%PUTAOTANGDEXUEQINGDENGDENG。

 

众赢彩票注册  8、BordelliSHIFA:QUGANJINGDEXIAOSHIGUAN(8×60mm)SAISHANGMIANSAI,MIEJUN。YONGMIEJUNDETUOZHINIURUXITUOSHIGUANXIEMIANSHANGDEJUNTAI,ZHICHENGNONGHOUJUNXUANYE。RANHOUYONGWUJUNXIGUANJIANGJUNXUANYEDIRUXIAOSHIGUANDIBU。MEIGUANYIDI,RANHOUZHUANDONGXIAOSHIGUANSHIJUNYEFENSANZAISHIGUANDIBUDEBISHANG。BIAOJIJUNMING。JIANGXIAOSHIGUANZHUANGZAI15×150mmDESHIGUANZHONG,ZAIDASHIGUANDIBUSHIXIANZHUANGSHANG1.5cmGAODE(P2O5HUOKOH),GUANKOUYONGDAIBOLIGUANDEXIANGPISAISAIJIN,LAFENG。JIANGDASHIGUANYUZHENKONGBENGLIANTONG,CHOUZHENKONGZHI0.1~0.2mmGONGZHUSHI,JIANGBOLIGUANRONGFENG,ZHISHIWENANCHUHUOBINGXIANGBAOCUN。

 

  YIQIYUCAILIAO1、XIEMIANJUNZHONG:SIZHUANGZHENJUN、JIAOMUJUN、YABAOGANJUNHEWUYABAOGANJUNGEYIZHI。

 

众赢彩票注册  2、JIEZHONGYONGJUYITAO。

 

  3、MIEJUNWUPIN:1ml、5mlXIGUAN,ZHANGDIGUAN,ANPINGGUAN,9mlWUJUNSHUISHIGUAN,SHATUGUAN,YETISHILA,TUOZHINIURU。QIZHONG,YETISHILADECHULIWEI1.5kg/cm2YALIMIEJUN1XIAOSHIHUO1kg/cm2YALIMIEJUN3CI,MEICI30FENZHONG。JIEZHEJIANCHASHIFOUCHEDIWUJUN,JIJIERUROUTANGZHONGJIANCHAYOUWUZAJUNSHENGZHANG。HOUFANGZAI60~80℃HONGKAOYIQUCHUSHUIFEN。TUOZHINIURUDECHULIWEI2000rpmLIXIN10FENZHONGTUOZHI,RANHOU0.5kg/cm2YALIMIEJUN20FENZHONG,HUOJIANXIEMIEJUN3CI,JINGJIANCHAWUJUNHOUBEIYONG。

 

  4、P2O5,WUSHUICaCl2GEYIPING。

 

众赢彩票注册  5、ZHENKONGGANZAOQI,ZHENKONGBENG。

 

众赢彩票注册  FANGFA1、YETISHILAFUGAIFA:QUDAIBAOCUNDEJUNZHONGXIEMIAN,YONG5mlWUJUNXIGUANXIANGNEIJIARUMIEJUNSHILA,YAOQIUYETISHILAGAOCHUJUNXIEMIAN1cmZUOYOU,RUOBUGOUCIGAODU,ZECHANGHUIYINQIXIEMIANCHOUGANXIANXIANG。

 

  2、SHATUGUANFA:YONG1mlWUJUNXIGUANXIQU0.2~0.5mlJUNXUANYE,DIRUSHATUGUANZHONG,CHONGFENHUNYUNHOU,JIANGSHATUGUANFANGRUZHENKONGGANZAOQINEI,CHOUGAN,ZHENKONGGANZAOQINEIXUFANGZHIWUSHUICaCl2。HOU,JIANGGANZAOHAODESHATUGUANQUCHU,XUNSUYUHUOYANSHANGLAFENGGUANKOU,BINGZAISHIWENXIAHUOBINGXIANGZHONGJINXINGBAOCUN。

 

众赢彩票注册  3、LENGDONGGANZAOBAOZANGFA:

 

众赢彩票注册  ①YONGMIEJUNZHANGDIGUANQUCHU2DI(YUE0.1ml)WUJUNTUOZHINIURU,ZHIYUWUJUNANPINGGUANZHONG,RANHOUYONGLINGYIZHIWUJUNDIGUANQUCHUDENGTIJIDENONGJUNYE,TONGYANGZHIYUCIANPINGGUANZHONG,CHONGFENHUNYUN。

 

  ②JIZHONGANPINGGUAN,ZHIYU100mlSHAOBEIZHONG,JIANGSHAOBEIZHUANGJINZHENKONGLENGDONGGANZAOQIDEZHONGZHAONEI,KAIJIBINGKAILENGQUESHUIHOU,SHIJUNYEZAI-20℃WENDUXIAXUNSUDONGJIECHENGGUTI,RANHOUCHOUZHENKONGSHISHUIFENSHENGHUA,2XIAOSHIHOU,KEYITINGZHILENGDONGBINGZAISHAOGAODEWENDUXIAJINXINGGANZAO,ZHIZHIQUANGANWEIZHI。

 

众赢彩票注册  ③HOUQUCHUANPINGGUAN,BINGJIANGQIYUZHENKONGBENGLIANJIE,ZAICHOUZHENKONGHOU,XUNSUJIANGANPINGGUANRONGFENG,HOUBINGXIANGBAOCUN。

 

  4、GANYOUGUANBAOZANGFA:ZAI5mlJUNZHONGBAOZANGGUANZHONG,JIANGDENGTIJIDEJUNXUANYEHE40%DEGANYOUCHONGFENHUNYUNHOU,YU-20℃BINGXIANGZHONGBAOCUN。

上海市百典仪器厂

上海市百典仪器厂

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